Services
Within the CEP, we offer the following scientific and technical services (partially in cooperation with the Tissue Bank):
Project consulting:
- Study design and planning, chose of model
- Validation of experimental models
- Macroscopic and histological examination through veterinary and human pathologic expertise in pathology of animal models
- ooperation projects for scientific publications and presentations
Tissue processing
- Paraffin embedding and preparing of tissue sections
- Frozen sections
- Hematoxylin-Eosin (HE) staining
- Special staining (upon request)
- Immunohistochemistry (IHC)
- In Situ-Hybridisation
- DNA-/RNA extraction in cooperation with the department of molecular pathology (upon request)
- Other Molecular pathological studies in cooperation with the department of molecular pathology (upon request)
- Laser microdissection (LMD, Leica)
- Archiving of tissue (currently only paraffin blocks)
- Light-sheet-fluorescence-microscopy
Autopsy services
- Small laboratory animals
- Other animals (only upon request and agreement)
Archiving and Digital processing
- Digital pathology
- Scanning and annotating of slides
- Computer based analysis (Definiens Tissue Studio®, Leica Aperio Algorithms, QuPath)
- Digital slide database (Aperio eSlide Manager, Leica)
Animal model tissue bank
- Preparation of tissue microarrays (TMA) of animal models
- Storage of normal tissue of frequently used mouse and rat models
Training
- Courses on tissue sampling and storage
- One-week-course on lab animal pathology as part of the PhD-program “medical life science and technology”
- Courses on general and special necropsy techniques (rodents)
- Theoretical seminars “Basics of animal pathology” on a regular basis
- Constant lectures “Basics of comparative pathology for experimental animal work” within the FELASA course at the ZPF
- Box “normal tissue and species associated diseases” for self-study (being set up at the moment, use for a fee/deposit)
Spatial Omics
o Spatial Transcriptomics (Xenium in situ, 10x Genomics)
- Single-cell and subcellular resolution
- Targeted method requiring the selection of gene panels. Currently, the parallel readout of 100s
of RNAs possible.
o Multiplexed antibody staining (CODEX/Phenocycler, Akoya Biosciences)
- Parallel readout of 10s to 100s of antibody stainings
o Both methods work with sections from FFPE and fresh-frozen tissue samples.